"Brain organoids are useful models for modeling brain diseases. However, long-term culture, high costs, and storage problems significantly limit their application in biomedical studies. Here, we establish a method using methylcellulose, ethylene glycol, DMSO, and Y27632 (termed MEDY) for the cryopreservation of diverse brain organoids or human brain tissue without disrupting the neural cytoarchitecture or functional activity."
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(24)00121-8
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(24)00121-8