Reproduction roundup! Link to many reproduction related articles in comment.
"'Brave New World' just got more real with the first mammal ever born from an unfertilized egg"
"Parthenogenesis does not happen naturally in mammals, and previous attempts ran into DNA glitches. Because mammalian conception needs genes that can only come from a male parent, the research team solved that by editing certain female genes with CRISPR until they were the same as if they had come from a male. They repeated the process with several eggs. These were then implanted into the female’s uterus and allowed to grow, and they went from embryonic to fetal stages to actual, living offspring, one of which was eventually able to produce its own offspring."
https://www.syfy.com/syfy-wire/parthenogenesis-has-been-achieved-in-mammals-for-the-first-time
"In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine."
https://www.pnas.org/doi/full/10.1073/pnas.2115248119
"'Brave New World' just got more real with the first mammal ever born from an unfertilized egg"
"Parthenogenesis does not happen naturally in mammals, and previous attempts ran into DNA glitches. Because mammalian conception needs genes that can only come from a male parent, the research team solved that by editing certain female genes with CRISPR until they were the same as if they had come from a male. They repeated the process with several eggs. These were then implanted into the female’s uterus and allowed to grow, and they went from embryonic to fetal stages to actual, living offspring, one of which was eventually able to produce its own offspring."
https://www.syfy.com/syfy-wire/parthenogenesis-has-been-achieved-in-mammals-for-the-first-time
"In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine."
https://www.pnas.org/doi/full/10.1073/pnas.2115248119